NAD+ 500 mg

Peptide Hubs NAD+ 500 mg: High-Dose Cellular Energy Coenzyme for Research

Peptide Hubs presents NAD+ 500 mg, a research-grade formulation of the essential coenzyme Nicotinamide Adenine Dinucleotide. Provided as a potent 500 mg lyophilized powder in a 2 mL vial, this product offers a concentrated resource for investigating cellular metabolism, energy production, and longevity pathways. NAD+ is a fundamental redox agent involved in hundreds of enzymatic reactions, serving as a critical electron carrier in mitochondrial ATP synthesis and as a required substrate for sirtuins and PARPs—enzymes governing DNA repair, gene expression, and cellular stress resistance. As emphasized in comprehensive reviews on NAD+ biology, declining cellular NAD+ levels are linked to age-related dysfunction, making its replenishment a key subject in metabolic and aging research. Peptide Hubs ensures pharmaceutical-grade purity for reliable, reproducible scientific inquiry into performance and recovery.

Effects of NAD+ 500 mg

NAD+ operates as a master regulator of cellular energetics and homeostasis. Its research effects are broad and systemic, impacting fundamental processes that underpin performance and health:

• Enhanced Cellular Energy (ATP) Production: As the primary electron acceptor in glycolysis and the Krebs cycle, and a core component of the electron transport chain, elevated NAD+ levels are studied for their potential to optimize mitochondrial efficiency and ATP output, which is crucial for muscular endurance and recovery.
• Activation of Longevity Pathways (Sirtuins): NAD+ is the sole substrate for sirtuin enzymes (SIRT1-7). Research investigates how boosting NAD+ can activate these proteins, which regulate mitochondrial biogenesis, antioxidant defenses, insulin sensitivity, and cellular repair, mimicking effects of caloric restriction.
• Improved DNA Repair & Genomic Stability: NAD+ fuels PARP enzymes that repair DNA damage. Studies examine its role in maintaining genomic integrity, especially under conditions of oxidative stress induced by intense exercise or environmental factors.
• Neuroprotection & Cognitive Function: Neurons are highly metabolically active and sensitive to NAD+ levels. Research explores NAD+'s potential in supporting brain energy metabolism, neurotransmitter synthesis, and resilience against excitotoxicity and age-related decline.
• Metabolic Regulation & Insulin Sensitivity: Through sirtuin activation, NAD+ is studied for its influence on glucose metabolism, lipid oxidation, and mitochondrial function in metabolic tissues like muscle and liver.

Recommended Dosage & Administration for Research

NAD+ is a research chemical, and all protocols are for laboratory study. The high-concentration 500 mg vial requires careful reconstitution. Adding 2 mL of bacteriostatic water or sterile saline yields a very concentrated 250 mg/mL solution. For most preclinical models, this is then further diluted for administration. Research dosing varies widely but is typically in the range of 100-500 mg per kg of body weight in animal studies, administered via intraperitoneal (IP) or intravenous (IV) routes to study systemic effects. In models focused on localized effects, subcutaneous or intramuscular routes are explored. Due to NAD+'s rapid metabolism, research protocols often utilize daily or every-other-day administration over cycles of 1-4 weeks to study cumulative effects on tissue NAD+ levels. Sterile technique is paramount, especially for IV or IP studies.

Potential Research Cycles & Experimental Stacking

NAD+ research spans from short, acute intervention studies to longer cycles (4-12 weeks) examining chronic adaptation. Given its foundational role, NAD+ is often studied in combination with other compounds that may synergize with or depend on its pathways. The following are research design concepts.

• For Anti-Aging & Cellular Renewal: Research may stack NAD+ with telomere-supporting peptides like Epitalon 50 mg or mitochondrial protectants like SS-31 50 mg to comprehensively target hallmarks of aging.
• For Metabolic & Fat Loss Studies: To investigate enhanced metabolism, it could be paired with compounds that increase energy expenditure, such as AOD 9604 5 mg, or appetite regulators like Semaglutide.
• For Cognitive & Neural Performance: Studies on brain health might combine it with neuropeptides like Selank (for anxiety modulation) or Pinealon 20 mg (for neural plasticity).
• For Muscle Recovery & Anabolism: Research could examine NAD+ alongside repair peptides like BPC 157 and growth factors like PEG MGF 5 mg to study the energy substrate's role in tissue repair.
• For Hormonal & Endocrine Support: In models of post-cycle recovery or hormonal optimization, it might be studied with peptides affecting the HPA/HPT axis, such as Kisspeptin 10 mg or during therapy with SERMs.

Possible Side Effects & Research Considerations

In research models, NAD+ is generally well-tolerated due to its endogenous nature. However, at high research doses, especially with rapid IV administration, transient side effects have been observed. These can include nausea, flushing, sensations of heat or itching, and changes in heart rate or blood pressure, likely related to its vasodilatory properties. A more significant research consideration is the potential for feedback inhibition of NAD+ biosynthetic pathways (the "NR/NMN salvage pathway") with chronic, high-dose exogenous administration, which could theoretically blunt natural production. The primary risks remain technical: improper sterile technique leading to infection, incorrect dosing, and the challenges of IV or IP administration requiring specialized surgical skills and ethical oversight.

Mechanism of Action & Research Significance

NAD+ is not a signaling molecule in the traditional sense; it is a vital cofactor. Its research significance lies in its consumption by three key classes of enzymes: 1) Dehydrogenases in energy metabolism (e.g., in mitochondria), where it is reduced to NADH to carry electrons. 2) Sirtuins (SIRTs), which are NAD+-dependent deacetylases that regulate stress response, metabolism, and epigenetics. 3) PARPs (Poly-ADP-ribose polymerases), which consume NAD+ to repair DNA strand breaks. Declining NAD+ with age or stress limits the activity of these systems. Research with high-dose NAD+ aims to determine if repletion can restore optimal function of these critical pathways, thereby improving cellular resilience, metabolic output, and repair capacity. This makes it a cornerstone compound for foundational healthspan and performance research.

Frequently Asked Questions (FAQ)